Tubes Pigment

Tubes Pigment Ive wrote my own chromatography experiment and i want to evaluate, in the terms of how to improve and maybe? what worked well. Here's my method: Procedure 1)Add a small amount of Pr...


Tubes Pigment

Tubes Pigment
Ive wrote my own chromatography experiment and i want to evaluate, in the terms of how to improve and maybe?

what worked well. Here's my method:

Procedure

1)Add a small amount of Propanone into the Mortar. Place a handful of nettle leaves into the Mortar
2)Grind the nettles in the propanone until you get a moderate amount of pigmented solution
3)Use the filter paper to filter the solution into a beaker.
4)Take a piece of Chromatography paper and draw a line 10mm from one of the ends. Mark a point in the centre of the line-this will be the pigment spot.
5)Use the micropipette to extract a sample of the solution and then place a drop onto the spot drawn as before. Let the drop dry, and then place a second small drop on the first. Repeat this process 5 times in order to build up a concentrated pigment in the small area.
6)Whilst preparing the pigment spot, pour the solvent into the boiling tube to a depth no more than 15mm.
7)Fold the opposite end of the Chromatography paper and suspend the Chromatography paper in the boiling tube.

A good outline.

If you have solvent to a depth of 15 mm, you need to have your spot placed higher, because you want it to be above the level of the solvent.

You should close off the end of the boiling tube with a stopper or some kind of sealing film. You should then leave the experimental setup standing in a place with no draughts and if possible a fairly uniform temperature.

You would improve the experiment enormously if you ran a parallel experiment using ethanol as solvent, to see whether this made any difference.

Good luck.

 
SkinCeuticals Pigment Regulator 6 Tubes Sample size
SkinCeuticals Pigment Regulator 6 Tubes Sample size
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